Algal cell disruption

Typical composition

Chlorella algae or different algae suspension in water 15% p/v.

The homogenization process

Homogenizer used: Panda NS1001L
Goal: more than 80% of cells destroyed
Pressure applied: 1000 bar x1 pass, 1000 bar x2 passes, 1200 bar x1 pass, 1400 bar x1 pass

The homogenization results:

Analysis method: Cells counted by an optical microscope before and after the high pressure treatment, magnification 400X, (dilution factor 20.000 μl water:100 μl cells suspension).

Results:

* Ten counts average value
** The cells suspension was cooled before the second pass.
*** The cells suspension was not cooled before the second pass.

Temperature of sample 5 before the first passage was about 10°C, temperature of sample 6 before the first passage was about 7°C.

Homogenizer:

The homogenizer used for the treatment is a machine designed for 1000- 1500 bar with VHP compression block for

• High abrasion
• High viscosity

One homogenizing stage; Knife edge design valve for cell disruption.